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What is a .fcs file?

A .fcs file is a Flow Cytometry Standard File (version 1.0-3.1). This one is a specialist format without an obvious everyday home.

How to open a .fcs file

  • The program that created it is by far the most reliable way to open it.
  • On Windows, use a hex viewer or the free TrID tool to identify what the file really is.
  • On Mac or Linux, running `file yourfile` in the terminal identifies most formats instantly.

The registry records this extension against Flow Cytometry Standard File, so software from that family is the most likely match.

How to convert a .fcs file

  • Identify the file first, then look for an export or Save As option in whatever program can read it.

MIME type

When a .fcs file travels over the web, servers label it with this MIME type:

application/vnd.isac.fcs

What's a MIME type?

The technical record

For archivists, developers and the merely curious: these are the PRONOM registry entries for .fcs.

Format nameVersionMIME typePUID
Flow Cytometry Standard File1.0-3.1 application/vnd.isac.fcsfmt/1737

Got another one?